Effective bioreduction of hexavalent chromium–contaminated water in fixed-film bioreactors

Abstract

Hexavalent chromium (Cr6+) contamination from a dolomite stone mine in Limpopo Province, South Africa, has resulted in extensive groundwater contamination. In order to circumvent any further negative environmental impact at this site, an effective and sustainable treatment strategy for the removal of up to 6.49 mg/ℓ Cr6+ from the groundwater was developed. Laboratory-scale, continuous up-flow bioreactors were constructed to evaluate reduction of Cr6+, with a residence time of 24 h, an efficiency porosity of 44% and a flow rate of 1.5 mℓ/min. Stoichiometrically balancing terminal electron acceptors in the feed water with a selected electron donor, directed reactor balance for complete Cr6+ reduction. The microbial community shifted in relative dominance during operation to establish an optimal metal-reducing community, including Enterobacter cloacae, Flavobacterium sp. and Ralstonia sp., which achieved 100% reduction.

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