Biolog for the determination of diversity in microbial communities

Abstract

Diversity and dynamics of microbial communities have been analysed by culture-dependent methods, which exclude the majority of fastidious microbes due to the selective nature of the media. Molecular methods have been used to determine diversity of microbial communities, but indicate the genetic complexity within a community. An alternative approach is to examine components of functional biodiversity (i.e. substrate utilisation), for which there exists a reasonable chance of detecting patterns, which could be related to the functional diversity of the species present in the community. In this study, different carbon source profiles were generated by inoculating Biolog GN and GP microtitre plates, with different dilutions of microbial communities. The high number of substrates utilised at the lower dilutions (10-1 and 10-2) indicated a high functional diversity in the communities tested. This, however, did not necessarily reflect the evenness of the functionality. Functional evenness of each species was reflected upon further dilution.

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