Characterisation of microorganisms responsible for EBPR in a sequencing batch reactor by using the 16S rDNA-DGGE method

Abstract

CCG CCG CGC GCG GCG GGC GGG GCG GGG GCA CGG GGG GCC TAC GGG AGG CAG CAG-3’), 102(5’-ATT ACC GCG GCT GCT GG-3’), F27 (5’-AGA GTT TGA TCC TGG CTC AG-3’), R1522 (5’-AAG GAG GTG ATC CAG CCG CA-3’), BSA, polymerase, dNTP were purchased from TaKaRa bio technology (Dalian) Co. Ltd, GoldView staining was purchased from Beijing SBS Genetech Co., Ltd. SBR operation methods At room temperature, the operation of a 3.5 ℓ SBR (3 ℓ water and 0.5 ℓ sludge) was conducted for 2 months to obtain PAOs responsible for EBPR. Activated sludge was inoculated and the reactor was operated with a 6.0 h cycle, consisting of a 2 min filling phase (with nitrogen bubbled for 2 min to assure anaerobic conditions during the feed), a 1.5 h anaerobic phase, a 4.0 h aerobic phase, an 0.3 h settling phase and a 5min withdrawal phase. After one cycle, 3 ℓ of the effluent was exchanged with an influent of the same amount and thus 7.0 h of the hydraulic retention time (HRT) was maintained. At the end of one cycle, 10 mℓ excess activated sludge was also wasted to maintain a sludge retention time (SRT) of 12.5 d.

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